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qpcr probesmaster kit  (Jena Bioscience)


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    Structured Review

    Jena Bioscience qpcr probesmaster kit
    Qpcr Probesmaster Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qpcr+probesmaster+kit/Direct+qPCR+ProbesMaster/pmc12702612-82-34-37
    Average 93 stars, based on 2 article reviews
    qpcr probesmaster kit - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Extraction:

    Article Title: Microbiome Dynamics in Early Life Stages of the Precious Mediterranean Red Coral Corallium rubrum
    Article Snippet: .. After collection, samples were directly put in 50 μL (larva and coral samples) or 100 μL (seawater filters) of extraction buffer of the direct qPCR ProbesMaster kit (Jena Bioscience) to lyse the cells and release DNA. .. Cell lysis was assisted by adding a Proteinase K solution (0.1 μL; 600 U/mL; Qiagen) and warming the samples at 65°C for 20 min before amplification of the 16S rRNA gene using the direct PCR.

    Article Title: Positive Interactions Under Ocean Warming and Acidification: Crustose Coralline Algae Holobionts Enhance Gorgonian Larval Settlement Under Climate Change
    Article Snippet: .. The CCA fragments of the respective aquaria and treatment were also collected and immediately frozen at −80°C, while larvae and settlers were directly put into 50 μL of the extraction buffer of the direct qPCR ProbesMaster kit (Jena Bioscience) and frozen for subsequent analysis of the associated microbiome. .. The quality of the sequences was assessed by visual inspection of the electropherograms using the Chromas software (Version 2.6.6; Technelysium Pty LTD, South Brisbane, Australia).

    Article Title: Microbiome Dynamics in Early Life Stages of the Precious Mediterranean Red Coral Corallium rubrum.
    Article Snippet: .. After collection, samples were directly put in 50 μL (larva and coral samples) or 100 μL (seawater filters) of extraction buffer of the direct qPCR ProbesMaster kit (Jena Bioscience) to lyse the cells and release DNA. .. Cell lysis was assisted by adding a Proteinase K solution (0.1 μL; 600 U/mL; Qiagen) and warming the samples at 65°C for 20 min before amplification of the 16S rRNA gene using the direct PCR.

    Real-time Polymerase Chain Reaction:

    Article Title: Microbiome Dynamics in Early Life Stages of the Precious Mediterranean Red Coral Corallium rubrum
    Article Snippet: .. After collection, samples were directly put in 50 μL (larva and coral samples) or 100 μL (seawater filters) of extraction buffer of the direct qPCR ProbesMaster kit (Jena Bioscience) to lyse the cells and release DNA. .. Cell lysis was assisted by adding a Proteinase K solution (0.1 μL; 600 U/mL; Qiagen) and warming the samples at 65°C for 20 min before amplification of the 16S rRNA gene using the direct PCR.

    Article Title: Positive Interactions Under Ocean Warming and Acidification: Crustose Coralline Algae Holobionts Enhance Gorgonian Larval Settlement Under Climate Change
    Article Snippet: .. The CCA fragments of the respective aquaria and treatment were also collected and immediately frozen at −80°C, while larvae and settlers were directly put into 50 μL of the extraction buffer of the direct qPCR ProbesMaster kit (Jena Bioscience) and frozen for subsequent analysis of the associated microbiome. .. The quality of the sequences was assessed by visual inspection of the electropherograms using the Chromas software (Version 2.6.6; Technelysium Pty LTD, South Brisbane, Australia).

    Article Title: Microbiome Dynamics in Early Life Stages of the Precious Mediterranean Red Coral Corallium rubrum.
    Article Snippet: Cell lysis was assisted by adding a Proteinase K solution (0.1 μL; 600 U/mL; Qiagen) and warming the samples at 65°C for 20 min before amplification of the 16S rRNA gene using the direct PCR. .. For each sample, three replicate PCRs were done on the V3- V4 region of the 16S rRNA gene using the direct qPCR ProbesMaster kit (Jena Bioscience) and the forward and reverse primers 341F 5′- CCTACGGGNGGCWGCAG- 3′ and 785R 5′- GACTACHVGGGTATCTAATCC- 3′, respectively (Klindworth et al. 2013). ..

    Article Title: Microbiome Dynamics in Early Life Stages of the Precious Mediterranean Red Coral Corallium rubrum.
    Article Snippet: .. After collection, samples were directly put in 50 μL (larva and coral samples) or 100 μL (seawater filters) of extraction buffer of the direct qPCR ProbesMaster kit (Jena Bioscience) to lyse the cells and release DNA. .. Cell lysis was assisted by adding a Proteinase K solution (0.1 μL; 600 U/mL; Qiagen) and warming the samples at 65°C for 20 min before amplification of the 16S rRNA gene using the direct PCR.

    Article Title: Microbiome Dynamics in Early Life Stages of the Precious Mediterranean Red Coral Corallium rubrum
    Article Snippet: Cell lysis was assisted by adding a Proteinase K solution (0.1 μL; 600 U/mL; Qiagen) and warming the samples at 65°C for 20 min before amplification of the 16S rRNA gene using the direct PCR. .. For each sample, three replicate PCRs were done on the V3‐V4 region of the 16S rRNA gene using the direct qPCR ProbesMaster kit (Jena Bioscience) and the forward and reverse primers 341F 5′‐CCTACGGGNGGCWGCAG‐3′ and 785R 5′‐GACTACHVGGGTATCTAATCC‐3′, respectively (Klindworth et al. ). ..

    other:

    Article Title: Positive Interactions Under Ocean Warming and Acidification: Crustose Coralline Algae Holobionts Enhance Gorgonian Larval Settlement Under Climate Change
    Article Snippet: The three PCR reactions per sample were pooled and amplicons were purified using AMPure XP beads (Beckman Coulter).



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